Intensive Rehabilitation Enhances Lymphocyte BDNF-TrkB Signaling in Patients With Parkinson’s Disease.
by Summer
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Background In the animal combined and Human study, we have previously found that treatment of 5-day increase the plasticity of cortical also facilitates brain – derived neurotrophic factor ( BDNF ) – receptor tyrosine kinase B (TrkB) signaling and increased activated TrkB and N-methyl-d-aspartate receptor (NMDAR) association in both the cortex and peripheral lymphocytes.
Patients with Parkinson’s disease (PD), in general, show decreased cortical plasticity, as shown by electrophysiological and behavioral studies. Here, we tested the hypothesis that exercise programs that improve motor function and appears to slow the progression of symptoms can improve BDNF -TrkB signals in lymphocytes. Methods A total of 16 patients with PD experience 4 -week intensive multidisciplinary rehabilitation treatment (MIRT), which includes aerobic exercise and physical and occupational therapy.
Blood was collected before and after 2 and 4 MIRT week. Lymphocytes isolated to check the BDNF -TrkB signaling caused by incubation with recombinant Human BDNF . TrkB signaling complex, extracellular-signal-regulated kinase-2 and protein-kinase-B immunoprecipitated; immunocomplexes contents determined by Western blotting. Results After MIRT, all patients showed improvements in motor function. TrkB interaction with NMDAR and BDNF -TrkB signaling increases in peripheral lymphocyte receptor, intracellular mediators, and the downstream level.
The decrements in the Unified Parkinson’s Disease Rating Scale II (UPDRSII) and total scores were significantly correlated with the increase in receptor TrkB signaling, intracellular mediators, and the level of interaction of NMDAR. Conclusion The significant correlation between reduced UPDRS score and change the activity of lymphocytes demonstrated that improved BDNF -TrkB signaling in lymphocytes and reduce the severity of symptoms of PD may be related.
Intensive Rehabilitation Enhances Lymphocyte BDNF-TrkB Signaling in Patients With Parkinson’s Disease.
Late pulses like receptor 4 inversely attenuating the effects of Chinese herbal Xiao-Qing-Long-Tang on allergen-induced nerve growth factor and thymic stromal lymphopoietin.4
Xiao-Qing-Long-Tang (XQLT) is known to regulate the immune allergic reaction. The purpose of this study was to investigate the effects of XQLT on allergen-induced cytokine and related signaling pathways. A rat model of acute allergic used to investigate the effects of XQLT on nerve growth factor (NGF) during an allergic reaction, while Human pulmonary alveolar epithelial cells (HPAEpiCs) was used to investigate the effects of XQLT on Dermatophagoides pteronyssinus group 2 ( der p 2) -induced NGF, p75 neurotrophin receptor (p75NTR) and thymic stromal lymphopoietin (TSLP) expression. XQLT shown to inhibit allergic reactions associated NGF- and p75NTR in a mouse model.
XQLT also reduce the expression of Toll-like receptor 4 (TLR 4 ) in the lungs of rat models. XQLT inhibit Der p 2-induced NGF, TSLP and p75NTR expression in cell lines HPAEpiC. The use recombinant TLR soluble 4 (sTLR 4 ) in the competitive test XQLT partially attenuated inhibitory effect on NGF, TSLP and p75NTR expression in HPAEpiC cells. XQLT inhibitory effect on Ser536 phosphorylation of p65 (nuclear factor-kB; NF-kB), a TLR 4 the impact factor, is also attenuated by sTLR 4 .
Description: Neurotrophin-4 (NT-4), also known as neurotrophin-5 (NT-5), is a protein that in humans is encoded by the NTF4 gene. This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood.
Description: Neurotrophin-4 Human Recombinant produced in E.Coli is a noncovalently linked homodimer, non-glycosylated polypeptide chain containing 2 x 130 amino acids (81-210 amino acids) and having a total molecular mass of 28 kDa. ;The NT-4 is purified by proprietary chromatographic techniques.
Recombinant Human NTF3/ Neurotrophin 3 Protein, Untagged, E.coli-10ug
Description: Neurotrophin-1 Human Recombinant (28-225) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 199 amino acids and having a molecular mass of 22kDa.;The NNT-1 is purified by proprietary chromatographic techniques.
Description: Neurotrophin-3 Human Recombinant produced in E.Coli is a non-glycosylated and non-covalently linked homodimer, containing 2x119 amino acid chains, having a total Mw of 27.2 kDa.;The NT-3 is purified by proprietary chromatographic techniques.
Description: Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors and is structurally related to beta -NGF, BDNF and NT-4. The NT3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT3.The amino acid sequences of mature human, murine and rat NT-3 are identical. NT-3 selectively promotes the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems.
Genorise® Recombinant Equine Neurotrophin-4 protein
Description: Neurotrophin-3 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 119 amino acids and having a molecular mass of 13.6kDa. ;The NT-3 is purified by proprietary chromatographic techniques.
Description: Recombinant NT-4 is a disulfide-linked homodimer protein consisting of two 131 amino acid residues, and migrates as an approximately 29 kDa protein under non-reducing and as 14 kDa under reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Neurotrophin-4 mature chain was expressed in E. coli.
Description: Recombinant NT-4 is a disulfide-linked homodimer protein consisting of two 131 amino acid residues, and migrates as an approximately 29 kDa protein under non-reducing and as 14 kDa under reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Neurotrophin-4 mature chain was expressed in E. coli.
Genorise® Recombinant Equine Neurotrophin-3 protein
Description: NTF3 is a member of the neurotrophin family that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.
Description: NTF3 is a member of the neurotrophin family that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.
Description: The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.
Description: A competitive ELISA for quantitative measurement of Human Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
In conclusion, XQLT hampered Der p allergen-induced NGF, p75NTR and TSLP expression. This inhibition is attenuated by sTLR 4 . The working mechanism of XQLT may be correlated with TLR 4 , the main receptors in the innate immune system. The findings of this study can focus on the pharmacological target search XQLT to TLR 4 , which is important in the presentation of allergens path.
