Neurotrophin gene augmentation by electrotransfer to improve cochlear implant hearing outcomes.
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Overview outlines the development of therapeutics based on DNA for the treatment of hearing loss, and in particular, considers the potential for utilizing the properties recombinant neurotrophins to improve hearing cochlea (spiral ganglion) survival of neurons and repair , This potential to reduce the spiral ganglion neuron death and indeed re-grow the auditory nerve fibers have been the subject of pre-clinical evaluation sufficient for decades with a view of increasing the neural interface with cochlear implants.
It provides the context for the discussion on the development of novel means of using a cochlear implant electrode arrays for gene electrotransfer. mesenchymal perilymphatic compartments that line the cochlea can be selectively transfected with the (naked) plasmid DNA using various – based gene electrotransfer, called ‘near-field of electroporation’. This technology is able to drive the expression of brain derived neurotrophic factor ( BDNF ) in a rabbit model trial hearing, causing the regrowth of peripheral spiral ganglion cell neurites toward mesenchymla, and thus be close to the cochlear implant electrodes in the scala tympani.
This was associated with functional improvement of the cochlear implant neural interface (lower the recruitment threshold of nerve and extended dynamic range, measured using electrically – Evoked auditory brain stem response). Basis for the near-field of electroporation efficiency arises from compression of the electric field is close to conspire against cochlear implant electrodes. The area close to the array with the highest field strength is closely related to the distribution of bioreporter cells (adherent Human embryonic kidney (HEK293)) revealed the reporter green fluorescent protein (GFP) gene electrotransfer following.
Neurotrophin gene augmentation by electrotransfer to improve cochlear implant hearing outcomes.
Optimizing the parameters of gene electrotransfer using a cell-based model is closely correlated with in vitro and in vivo gene delivery results cochlea. Migration of cochlear implant electrode array-based platform that gene electrotransfer to a clinical trial for neurotrophin the cochlear implant-based improvement is supported by the availability of regulatory compliant plasmid backbone DNA mini-new (pFAR 4 ;. Free of antibiotic resistance plasmids v 4 ) that can be used to package ‘ human to materialize’ neurotrophin expression cassette.
A reporter cassette packed into pFAR 4 produced by expression of GFP prominent in the basal turn of guinea pig scalae perilymphatic. More broadly, the near-field of gene electrotransfer may lend itself to a spectrum of potential applications of DNA therapeutic benefits of titratable, local, naked DNA delivery, for enlargement of genes, gene regulation targeted strategy, or gene substitution. WST-1 test results revealed that compared with group / R EGD, the survival rate of cells was significantly higher in OGD / R group and lower MIF in OGD / R with ISO-1 group.
Description: A competitive ELISA for quantitative measurement of Human Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Quantitativesandwich ELISA kit for measuring Human Neurotrophin 4, NT-4 in samples from serum, plasma, cell culture supernates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Human Neurotrophin 4, NT-4 in samples from serum, plasma, cell culture supernates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Neurotrophin-4 in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Neurotrophin 4 (NT4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Neurotrophin 4 (NT4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neurotrophin 4 (NT4) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neurotrophin 4 (NT4) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neurotrophin 4 (NT4) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neurotrophin 4 (NT4) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Neurotrophin 4 (NT4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Recombinant NT-3 is a disulfide-linked homodimer protein consisting of two 120 amino acid residue subunits, and migrates as an approximately 27 kDa protein under non-reducing and as 14 kDa under reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Neurotrophin-3 mature chain was expressed in E. coli.
Description: Recombinant NT-3 is a disulfide-linked homodimer protein consisting of two 120 amino acid residue subunits, and migrates as an approximately 27 kDa protein under non-reducing and as 14 kDa under reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Neurotrophin-3 mature chain was expressed in E. coli.
Description: Neurotrophin-4 Human Recombinant produced in E.Coli is a noncovalently linked homodimer, non-glycosylated polypeptide chain containing 2 x 130 amino acids (81-210 amino acids) and having a total molecular mass of 28 kDa. ;The NT-4 is purified by proprietary chromatographic techniques.
Description: A sandwich ELISA kit for detection of Neurotrophin 4 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: Neurotrophin-4 (NT-4), also known as neurotrophin-5 (NT-5), is a protein that in humans is encoded by the NTF4 gene. This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood.
Rabbit anti human Neurotrophin-4 Antibody (Biotin)
Description: A competitive ELISA for quantitative measurement of Rat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Quantitativesandwich ELISA kit for measuring Rat Neurotrophin 4, NT-4 in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Rat Neurotrophin 4, NT-4 in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A competitive ELISA for quantitative measurement of Mouse Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
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Western blot assay and immunocytochemistry results revealed that the expression levels of BDNF , Bcl2, and MAP2 are significantly higher, and the level of expression of Caspase-3 and Bax were significantly lowered in the group MIF than in OGD / R group. the level of expression of BDNF , Bcl2, and MAP2 significantly lowered, and the level of expression of Caspase-3 and Bax were significantly higher in group 1 than in the ISO / OGD group R. MIF administration promoted cell survival nerves and causes a high level of expression of BDNF , MAP2, and Bcl-2 (anti-apoptotic) and the low expression levels of Caspase-3 and Bax (pro-apoptotic) in OGD / R models. this re
