Adsorption/Desorption Transition of RecombinantHumanNeurotrophin4: Physicochemical Characterization.
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Bulk physicochemical properties neurotrophin 4 (NT- 4 ) in the electrolyte solution and the adsorption / desorption on / from the mica surface has been studied by using dynamic scattering light (DLS), microelectrophoresis, depletion engineering solution (enzyme-linked immunosorbent assay, ELISA), and AFM imaging. Our study presents the determination of diffusion coefficients, hydrodynamic diameter, electrophoretic mobility and isoelectric point of NT- 4 under various ionic strength and pH conditions.
The size of NT- 4 homodimer to the ionic strength of 0.015 M is substantially independent of pH and equal to 5.1 nm. It has been found that the total cost of the electrokinetic per NT- 4 molecule is equal to zero for all ionic strengths studied at pH 8.1, which was identified as the isoelectric point (IEP). Protein adsorption / desorption on / from the mica surface was examined as a function of the ionic strength and pH. Kinetics neurotrophin the adsorption / desorption evaluated at pH 3.5, 7. 4 , and 11 by direct AFM imaging and ELISA techniques.
Increased monotonous in maximum coverage absorbed NT- 4 molecules with ionic strength (up to 5.5 mg / m2) was observed at pH 3.5. These results are interpreted in terms of theoretical models postulated an irreversible adsorption of proteins regulated by random sequential adsorption (RSA). Our measurements show the important role of the ionic strength, pH, and the composition of the electrolyte in the lateral electrostatic interactions between charged different NT- 4 molecule. The transition between the adsorption / desorption was found to area high pH and low surface concentration of adsorbed neurotrophin molecules at constant ionic strength.
In addition, the results presented in this work show that the adsorption behavior of neurotrophin molecules can be regulated by electrostatic interactions intrasolvent produce aggregation process. polyvalent understanding of neurotrophin interaction may affect the reversibility / irreversibility of the adsorption, and therefore they may be useful to obtain a protein coating that is ordered, targeting the future development of drug delivery systems to treat neurodegenerative diseases.
Adsorption/Desorption Transition of RecombinantHumanNeurotrophin4: Physicochemical Characterization.
Over-expression of neurotrophin 3 in human aortic valve calcification disease affected induce osteogenic response through Trk-Akt pathway.
Calcific aortic valve disease (CAVD) is a leading cardiovascular disorders in the elderly. While the aortic valve interstitial cells (AVICs) are master cells that express osteogenic mediator, the molecular mechanisms that mediate osteogenic response AVIC is not fully understood. The purpose of this study to identify the factors pro-osteogenic in human AVICs influenced by CAVD.
RESULTS Microarray analysis identified 11 genes up-regulated in diseased valve AVICs. Among these genes, mRNA levels of neurotrophin 3 (NT3) increased by 2-fold. a higher level of NT3 protein in diseased aortic valve and AVICs illness confirmed by immunofluorescent staining and immunoblotting, respectively. Exposure AVICs normal valve recombinant human NT3 (0.025-0.10μg / ml) up-regulated the production of Runx2, TGF-β1 and BMP-2 in a dose-dependent fashion.
NT3 also promote the formation of calcium deposits. Pro-osteogenic effect of the NT3 is not affected by the neutralization of the Toll-like receptor 2 or 4. Interestingly, mRNA that encodes nerve growth factor receptor (TrkA, TrkB, TrkC and p75 NTR) AVICs detected in humans.
Description: Recombinant NT-4 is a disulfide-linked homodimer protein consisting of two 131 amino acid residues, and migrates as an approximately 29 kDa protein under non-reducing and as 14 kDa under reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Neurotrophin-4 mature chain was expressed in E. coli.
Description: Recombinant NT-4 is a disulfide-linked homodimer protein consisting of two 131 amino acid residues, and migrates as an approximately 29 kDa protein under non-reducing and as 14 kDa under reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Neurotrophin-4 mature chain was expressed in E. coli.
Description: Neurotrophin-4 Human Recombinant produced in E.Coli is a noncovalently linked homodimer, non-glycosylated polypeptide chain containing 2 x 130 amino acids (81-210 amino acids) and having a total molecular mass of 28 kDa. ;The NT-4 is purified by proprietary chromatographic techniques.
Description: Interleukin-4 Human Recombinant produced in yeast is a single, glycosylated polypeptide chain containing 129 amino acids.;The IL-4 is purified by proprietary chromatographic techniques.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Neurotrophin 4 (NT4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: A competitive ELISA for quantitative measurement of Human Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Quantitativesandwich ELISA kit for measuring Human Neurotrophin 4, NT-4 in samples from serum, plasma, cell culture supernates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Human Neurotrophin 4, NT-4 in samples from serum, plasma, cell culture supernates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: 4-1BB Receptor, a member of the TNF superfamily of receptors, is mainly expressed on the surface of a variety of T cells, but also found in B cells, monocytes, and various transformed cell lines. 4-1BB Receptor binds to 4-1BBL to provide a co-stimulatory signal for T lymphocytes. Signaling by 4-1BB Receptor has been implicated in the antigen-presentation process and generation of cytotoxic T cells. The human 4-1BB Receptor gene codes for a 255 amino acid type I transmembrane protein containing a 17 amino acid N-terminal signal sequence, a 169 amino acid extracellular domain, a 27 amino acid transmembrane domain and a 42 amino acid cytoplasmic domain. Recombinant human soluble 4-1BB Receptor is a 167 amino acid polypeptide (17.7 kDa), which contains the cysteine rich TNFR-like extracellular domain of 4-1BB Receptor.
Description: PF-4 is a CXC chemokine that is expressed in megakaryocytes and stored in the α-granules of platelets. PF-4 is chemotactic towards neutrophils and monocytes and has been shown to inhibit angiogenesis. Recombinant human PF-4 is a 7.8 kDa protein containing 70 amino acid residues, including the four highly conserved residues present in CXC chemokines.
Description: A sandwich quantitative ELISA assay kit for detection of Human Neurotrophin 4 (NT4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Neurotrophin 4 (NT4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Neurotrophin-4 in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neurotrophin 4 (NT4) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neurotrophin 4 (NT4) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neurotrophin 4 (NT4) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neurotrophin 4 (NT4) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Neurotrophin 4 (NT4) in samples from Serum, plasma, tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Neurotrophin 4 (NT4) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: IL-4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is a key regulator in humoral and adaptive immunity. Feline IL-4 Recombinant Protein is purified interleukin-4 produced in yeast.
Description: A competitive ELISA for quantitative measurement of Goat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Neurotrophin 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
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Trk receptor inhibition markedly reduces the effect of NT3 on Runx2, TGF-β1 and the production of BMP-2, the formation of calcium deposits and Akt phosphorylation. Furthermore, inhibition of Akt also reduces pro-osteogenic effect of NT3.
