Bulk physicochemical properties neurotrophin 4 (NT- 4 ) in the electrolyte solution and the adsorption / desorption on / from the mica surface has been studied by using dynamic scattering light (DLS), microelectrophoresis, depletion engineering solution (enzyme-linked immunosorbent assay, ELISA), and AFM imaging. Our study presents the determination of diffusion coefficients, hydrodynamic diameter, electrophoretic mobility and isoelectric point of NT- 4 under various ionic strength and pH conditions.

The size of NT- 4 homodimer to the ionic strength of 0.015 M is substantially independent of pH and equal to 5.1 nm. It has been found that the total cost of the electrokinetic per NT- 4 molecule is equal to zero for all ionic strengths studied at pH 8.1, which was identified as the isoelectric point (IEP). Protein adsorption / desorption on / from the mica surface was examined as a function of the ionic strength and pH. Kinetics neurotrophin the adsorption / desorption evaluated at pH 3.5, 7. 4 , and 11 by direct AFM imaging and ELISA techniques.

Increased monotonous in maximum coverage absorbed NT- 4 molecules with ionic strength (up to 5.5 mg / m2) was observed at pH 3.5. These results are interpreted in terms of theoretical models postulated an irreversible adsorption of proteins regulated by random sequential adsorption (RSA). Our measurements show the important role of the ionic strength, pH, and the composition of the electrolyte in the lateral electrostatic interactions between charged different NT- 4 molecule. The transition between the adsorption / desorption was found to area high pH and low surface concentration of adsorbed neurotrophin molecules at constant ionic strength.

In addition, the results presented in this work show that the adsorption behavior of neurotrophin molecules can be regulated by electrostatic interactions intrasolvent produce aggregation process. polyvalent understanding of neurotrophin interaction may affect the reversibility / irreversibility of the adsorption, and therefore they may be useful to obtain a protein coating that is ordered, targeting the future development of drug delivery systems to treat neurodegenerative diseases.

Adsorption/Desorption Transition of <em>Recombinant</em> <em>Human</em> <em>Neurotrophin</em> <em>4</em>: Physicochemical Characterization.
Adsorption/Desorption Transition of Recombinant Human Neurotrophin 4: Physicochemical Characterization.

Over-expression of neurotrophin 3 in human aortic valve calcification disease affected induce osteogenic response through Trk-Akt pathway.

Calcific aortic valve disease (CAVD) is a leading cardiovascular disorders in the elderly. While the aortic valve interstitial cells (AVICs) are master cells that express osteogenic mediator, the molecular mechanisms that mediate osteogenic response AVIC is not fully understood. The purpose of this study to identify the factors pro-osteogenic in human AVICs influenced by CAVD.


RESULTS
Microarray analysis identified 11 genes up-regulated in diseased valve AVICs. Among these genes, mRNA levels of neurotrophin 3 (NT3) increased by 2-fold. a higher level of NT3 protein in diseased aortic valve and AVICs illness confirmed by immunofluorescent staining and immunoblotting, respectively. Exposure AVICs normal valve recombinant human NT3 (0.025-0.10μg / ml) up-regulated the production of Runx2, TGF-β1 and BMP-2 in a dose-dependent fashion.

NT3 also promote the formation of calcium deposits. Pro-osteogenic effect of the NT3 is not affected by the neutralization of the Toll-like receptor 2 or 4. Interestingly, mRNA that encodes nerve growth factor receptor (TrkA, TrkB, TrkC and p75 NTR) AVICs detected in humans.

Recombinant Human Neurotrophin-4

7-01304 10µg Ask for price

Recombinant Human Neurotrophin-4

7-01305 1mg Ask for price

Recombinant Human Neurotrophin-4

cyt-626-10g 10µg
EUR 145

Recombinant Human Neurotrophin-4

cyt-626-1mg 1mg
EUR 4680

Recombinant Human Neurotrophin-4

cyt-626-2g 2µg
EUR 60

Neurotrophin-4, recombinant (Human)

033-30 10 μg
EUR 390.96

Neurotrophin-4 Human Recombinant

rAP-2701 Inquiry Ask for price

Recombinant Human Neurotrophin-4 (NT-4)

HEOPP-1405 10ug Ask for price

Recombinant Human Neurotrophin-4(NTF4)

AP72096 1mg
EUR 1978

Recombinant Human Neurotrophin-3

7-01294 2µg Ask for price

Recombinant Human Neurotrophin-3

7-01295 10µg Ask for price

Recombinant Human Neurotrophin-3

7-01296 1mg Ask for price

Recombinant Human Neurotrophin-1

cyt-869-10g 10µg
EUR 145

Recombinant Human Neurotrophin-1

cyt-869-1mg 1mg
EUR 4680

Recombinant Human Neurotrophin-1

cyt-869-2g 2µg
EUR 60

Recombinant Human Neurotrophin-3

cyt-257-10g 10µg
EUR 145

Recombinant Human Neurotrophin-3

cyt-257-1mg 1mg
EUR 4680

Recombinant Human Neurotrophin-3

cyt-257-2g 2µg
EUR 60

Recombinant Human Neurotrophin-4 (NTF4), partial

CSB-EP016121HU1 1830 mg Ask for price

Neurotrophin-3, recombinant (Human)

033-29 10 μg
EUR 390.96

NT-4 Neurotrophin-4 Human Recombinant Protein

PROTP34130 Regular: 10ug
EUR 380.4
Description: Neurotrophin-4 Human Recombinant produced in E.Coli is a noncovalently linked homodimer, non-glycosylated polypeptide chain containing 2 x 130 amino acids (81-210 amino acids) and having a total molecular mass of 28 kDa. ;The NT-4 is purified by proprietary chromatographic techniques.

Human Neurotrophin-3 (Recombinant)

22060430-1 2 µg
EUR 146.42

Neurotrophin-3 Human Recombinant

rAP-2697 Inquiry Ask for price

Neurotrophin-1 Human Recombinant

rAP-2707 Inquiry Ask for price

Recombinant Human Neurotrophin-3/NT3

AP74825 1mg
EUR 3209

Recombinant Human Neurotrophin-3/NT3

C079-10ug 10ug
EUR 242.4
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2.

Recombinant Human Neurotrophin-3/NT3

C079-1mg 1mg
EUR 2739.6
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2.

Recombinant Human Neurotrophin-3/NT3

C079-500ug 500ug
EUR 1910.4
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2.

Recombinant Human Neurotrophin-3/NT3

C079-50ug 50ug
EUR 595.2
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2.

Recombinant Human Neurotrophin-3 (NTF3)

CSB-BP016120HU 7886 mg Ask for price

Recombinant Human Neurotrophin-3 (NTF3)

CSB-EP016120HU 7990 mg Ask for price

Recombinant Human Neurotrophin-3 (NTF3)

CSB-MP016120HU 7843 mg Ask for price

Recombinant Human Neurotrophin-3 (NTF3)

RPC27838-100ug 100ug
EUR 1463

Recombinant Human Neurotrophin-3 (NTF3)

RPC27838-1mg 1mg
EUR 6879.6

Recombinant Human Neurotrophin-3 (NTF3)

RPC27838-20ug 20ug
EUR 550

Recombinant Human Neurotrophin-3 (NTF3)

RPC28701-100ug 100ug
EUR 1170.4

Recombinant Human Neurotrophin-3 (NTF3)

RPC28701-1mg 1mg
EUR 3252

Recombinant Human Neurotrophin-3 (NTF3)

RPC28701-20ug 20ug
EUR 440

Recombinant Human Neurotrophin-3 (NTF3)

RPC28729-100ug 100ug
EUR 528

Recombinant Human Neurotrophin-3 (NTF3)

RPC28729-1mg 1mg
EUR 2412.5

Recombinant Human Neurotrophin-3 (NTF3)

RPC28729-20ug 20ug
EUR 295.7

Recombinant Neurotrophin 4 (NT4)

RPU56286-100ug 100ug
EUR 775.5

Recombinant Neurotrophin 4 (NT4)

RPU56286-1mg 1mg
EUR 3057.6

Recombinant Neurotrophin 4 (NT4)

RPU56286-50ug 50ug
EUR 470.4

Recombinant Neurotrophin 4 (NT4)

RPU59108-100ug 100ug
EUR 1044.4

Recombinant Neurotrophin 4 (NT4)

RPU59108-1mg 1mg
EUR 4083.3

Recombinant Neurotrophin 4 (NT4)

RPU59108-50ug 50ug
EUR 652.6

Recombinant Neurotrophin 4 (NT4)

RPU53898-100ug 100ug
EUR 566.5

Recombinant Neurotrophin 4 (NT4)

RPU53898-1mg 1mg
EUR 2511.6

Recombinant Neurotrophin 4 (NT4)

RPU53898-50ug 50ug
EUR 455.4

Recombinant Neurotrophin 4 (NT4)

RPA107Hu01 10ug
EUR 184

Recombinant Neurotrophin 4 (NT4)

4-RPA107Hu01
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Description: Recombinant Human Neurotrophin 4 expressed in: E.coli

Recombinant Neurotrophin 4 (NT4)

RPA107Mu01 10ug
EUR 179

Recombinant Neurotrophin 4 (NT4)

4-RPA107Mu01
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Description: Recombinant Mouse Neurotrophin 4 expressed in: E.coli

Recombinant Neurotrophin 4 (NT4)

RPA107Ra01 10ug
EUR 196

Recombinant Neurotrophin 4 (NT4)

RPU43034-100ug 100ug
EUR 552.2

Recombinant Neurotrophin 4 (NT4)

RPU43034-1mg 1mg
EUR 2446.6

Recombinant Neurotrophin 4 (NT4)

RPU43034-50ug 50ug
EUR 443.3

Recombinant Human Neurotrophin-4 protein(NTF4) (Active)

AP73547 1mg
EUR 6829

Recombinant Human Neurotrophin-4 protein (NTF4) (Active)

CSB-AP002831HU 8451 mg Ask for price

Recombinant Human Precursor Neurotrophin-3

7-01297 2µg Ask for price

Recombinant Human Precursor Neurotrophin-3

7-01298 10µg Ask for price

Recombinant Human Precursor Neurotrophin-3

7-01299 1mg Ask for price

Recombinant Human Neurotrophin-3 (NTF3) (Active)

CSB-AP003801HU 8580 mg Ask for price

Recombinant Human NT-3/Neurotrophin-3 Protein

E40KMP1632 20ug
EUR 495

NNT1 Neurotrophin-1 Human Recombinant Protein

PROTQ9UBD9-1 Regular: 10ug
EUR 380.4
Description: Neurotrophin-1 Human Recombinant (28-225) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 199 amino acids and having a molecular mass of 22kDa.;The NNT-1 is purified by proprietary chromatographic techniques.

Neurotrophin-3, recombinant (Human) - I-125 Labeled

T-033-29 10 μCi
EUR 1145.88

NT-3 Neurotrophin-3 Human Recombinant Protein

PROTP20783 Regular: 10ug
EUR 380.4
Description: Neurotrophin-3 Human Recombinant produced in E.Coli is a non-glycosylated and non-covalently linked homodimer, containing 2x119 amino acid chains, having a total Mw of 27.2 kDa.;The NT-3 is purified by proprietary chromatographic techniques.

Recombinant Human Neurotrophin-3/NTF3 Protein (Active)

PKSH032803-10ug 10ug
EUR 178
Description: Human

Recombinant Human Neurotrophin-3/NTF3 Protein (Active)

PKSH032803-50ug 50ug
EUR 523
Description: Human

Recombinant Mouse Neurotrophin-3

7-01300 2µg Ask for price

Recombinant Mouse Neurotrophin-3

7-01301 10µg Ask for price

Recombinant Mouse Neurotrophin-3

7-01302 1mg Ask for price

Recombinant Mouse Neurotrophin-3

cyt-688-10g 10µg
EUR 145

Recombinant Mouse Neurotrophin-3

cyt-688-1mg 1mg
EUR 4680

Recombinant Mouse Neurotrophin-3

cyt-688-2g 2µg
EUR 60

Neurotrophin-3 Mouse Recombinant

rAP-2702 Inquiry Ask for price

Recombinant Human Neurotrophin-3 Polypeptide (a. a. 119)

DAG339 10ug
EUR 897.6

Recombinant Neurotrophin 3 (NT3)

RPU55489-100ug 100ug
EUR 728.2

Recombinant Neurotrophin 3 (NT3)

RPU55489-1mg 1mg
EUR 2870.4

Recombinant Neurotrophin 3 (NT3)

RPU55489-50ug 50ug
EUR 441.6

Recombinant Neurotrophin 3 (NT3)

RPU56113-100ug 100ug
EUR 554.4

Recombinant Neurotrophin 3 (NT3)

RPU56113-1mg 1mg
EUR 2184

Recombinant Neurotrophin 3 (NT3)

RPU56113-50ug 50ug
EUR 336

Recombinant Neurotrophin 3 (NT3)

RPU56654-100ug 100ug
EUR 470.4

Recombinant Neurotrophin 3 (NT3)

RPU56654-1mg 1mg
EUR 2184

Recombinant Neurotrophin 3 (NT3)

RPU56654-50ug 50ug
EUR 385

Recombinant Neurotrophin 3 (NT3)

RPA106Hu01 10ug
EUR 172

Recombinant Neurotrophin 3 (NT3)

4-RPA106Hu01
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Description: Recombinant Human Neurotrophin 3 expressed in: E.coli

Recombinant Neurotrophin 3 (NT3)

RPA106Hu02 10ug
EUR 172

Recombinant Neurotrophin 3 (NT3)

4-RPA106Hu02
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  • 100 ug
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  • 200 ug
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  • 5 mg
Description: Recombinant Human Neurotrophin 3 expressed in: E.coli

Recombinant Neurotrophin 3 (NT3)

RPA106Mu01 10ug
EUR 140

Recombinant Neurotrophin 3 (NT3)

RPA106Mu02 10ug
EUR 140

Recombinant Neurotrophin 3 (NT3)

RPA106Ra01 10ug
EUR 184

Recombinant Neurotrophin 3 (NT3)

4-RPA106Ra01
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  • 100 ug
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Description: Recombinant Rat Recombinant Neurotrophin 3 (NT3) expressed in: E.coli

Recombinant Neurotrophin 3 (NT3)

RPA106Ra02 10ug
EUR 140

Recombinant Neurotrophin 3 (NT3)

4-RPA106Ra02
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Description: Recombinant Rat Neurotrophin 3 expressed in: E.coli

Recombinant Neurotrophin 3 (NT3)

RPU43032-100ug 100ug
EUR 530.2

Recombinant Neurotrophin 3 (NT3)

RPU43032-1mg 1mg
EUR 2347.8

Recombinant Neurotrophin 3 (NT3)

RPU43032-50ug 50ug
EUR 425.7

Recombinant Neurotrophin 3 (NT3)

RPU43033-100ug 100ug
EUR 530.2

Recombinant Neurotrophin 3 (NT3)

RPU43033-1mg 1mg
EUR 2347.8

Recombinant Neurotrophin 3 (NT3)

RPU43033-50ug 50ug
EUR 425.7

Genorise® recombinant Sheep Neurotrophin 4

GR123022 5 µg
EUR 285

Neurotrophin-3 Recombinant Protein

91-065 0.05 mg
EUR 821.4
Description: Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors and is structurally related to beta -NGF, BDNF and NT-4. The NT3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT3.The amino acid sequences of mature human, murine and rat NT-3 are identical. NT-3 selectively promotes the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems.

Recombinant Human Neurotrophin-3/NGF-2/NT-3 Protein

RP00496 10 μg
EUR 286

Recombinant Human Neurotrophin 3/NTF3 protein (His tag)

PDEH100226-100ug 100ug
EUR 118
Description: Human

Recombinant Human Neurotrophin 3/NTF3 protein (His tag)

PDEH100226-20ug 20ug
EUR 68
Description: Human

Recombinant Rat Neurotrophin-3 (Ntf3)

CSB-EP016120RA 10360 mg Ask for price

Genorise® Recombinant Equine Neurotrophin-4 protein

GR104319 5 µg
EUR 285

Recombinant Human NT-3/Neurotrophin-3/NTF3 Protein, No Tag

E40KMH1479 20ug
EUR 495

Human Neurotrophin-3 Recombinant Protein Tag Free Lyophilized

IHUNT3RTFLY50UG each
EUR 988
Description: Human Neurotrophin-3 Recombinant Protein Tag Free Lyophilized

Genorise® recombinant Bovine Neurotrophin 4 protein

GR120051 5 µg
EUR 285

Genorise® recombinant Canine Neurotrophin 4 protein

GR122076 5 µg
EUR 285

Genorise® Recombinant feline Neurotrophin 4 (NT-4) protein

GR186036 5 mg
EUR 285

NT-3 Neurotrophin-3 Mouse Recombinant Protein

PROTP20181 Regular: 10ug
EUR 380.4
Description: Neurotrophin-3 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 119 amino acids and having a molecular mass of 13.6kDa. ;The NT-3 is purified by proprietary chromatographic techniques.

Recombinant Human NTF3/ Neurotrophin 3 Protein, Untagged, E.coli-10ug

QP1246-10ug 10ug
EUR 284.4

Recombinant Human NTF3/ Neurotrophin 3 Protein, Untagged, E.coli-1mg

QP1246-1mg 1mg
EUR 5942.4

Recombinant Human NTF3/ Neurotrophin 3 Protein, Untagged, E.coli-2ug

QP1246-2ug 2ug
EUR 163.2

Genorise® recombinant Sheep Neurotrophin 3

GR123021 5 µg
EUR 285

Recombinant Human NTF3/ Neurotrophin 3 Protein, Untagged, E.coli-100ug

QP1246-100ug 100ug
EUR 849.6

Recombinant Human NTF3/ Neurotrophin 3 Protein, Untagged, E.coli-500ug

QP1246-500ug 500ug
EUR 3645.6

Trk receptor inhibition markedly reduces the effect of NT3 on Runx2, TGF-β1 and the production of BMP-2, the formation of calcium deposits and Akt phosphorylation. Furthermore, inhibition of Akt also reduces pro-osteogenic effect of NT3.