Neuroinflammation play an important role in the pathogenesis of germinal matrix hemorrhage (GMH). neurotrophin – 4 (NT- 4 ) is a member of the neurotrophin family and interact with tropomyosin receptor kinase B (TrkB ). NT- 4 has been shown to provide the following neurological effects of cerebral ischemia. We aimed to determine the function of the nerves of NT- 4 -TrkB signaling, as well as the signaling cascade downstream of phosphatidylinositol-3-kinase (PI3K) / protein kinase B (Akt) / forkhead box protein O1 (FoxO1), the following GMH in neonatal rats.

GMH induced by intraparenchymal injection of bacterial collagenase (0.3 U) in P7 rat pup. A total of 163 pups were used in this study. Recombinant Human NT- 4 is given intranasally at 1 hour after injection of collagenase. Selective TrkB ANA-12 antagonist, selective PI3K inhibitor LY29 4 002, and FoxO1 activate CRISPR given intracerebroventricularly at 2 4 hours before NT- 4 treatment to investigate the underlying mechanisms. Short-term and long-term neurobehavioral assessment, immunofluorescence staining, Nissl’s staining and Western blot performed.

The expression of phosphorylated TrkB increased after GMH, reaching peak levels on day 3 after bleeding. TrkB receptors were observed in neurons, microglia, and astrocytes. Administration rh-NT- 4 induced phosphorylation of TrkB, the expression of PI3K and Akt phosphorylation. Meanwhile, the decrease in FoxO1 and IL-6 levels. Selective inhibition of TrkB / PI3K / Akt signaling in microglia increase expression levels FoxO1 and pro-inflammatory cytokines. FoxO1 activate CRISPR increased expression of IL-6, suggesting that perhaps FoxO1 potential inducer of pro-inflammatory factors.

These results demonstrate that the PI3K / Akt / FoxO1 downstream signaling pathways may TrkB activation. Rat pups treated with rh-NT- 4 performed better than vehicle-treated animals both short-term and long-term behavioral tests. These data indicate that rh-NT – 4 reducing the expression of pro-inflammatory cytokines, improve neurological function, attenuated neuroinflammation, and thus reduced post-hemorrhagic hydrocephalus after GMH by TrkB / PI3K / Akt / FoxO1 track. These results indicate that rh-NT- 4 could be a promising therapeutic strategy for repairing nerve inflammation and hydrocephalus after GMH or other similar brain injuries.

NT-<em>4</em> attenuates neuroinflammation via TrkB/PI3K/FoxO1 pathway after germinal matrix hemorrhage in neonatal rats.
NT-4 attenuates neuroinflammation via TrkB/PI3K/FoxO1 pathway after germinal matrix hemorrhage in neonatal rats.

Brain-derived neurotrophic factors mediate macrophage migration inhibitory factor to protect neurons against oxygen-glucose deficiency.

Macrophage migration inhibitory factor (MIF) is a chemokine that plays an important role in immune system function. Previous research suggests that MIF protect neurons under ischemic conditions. However, several studies reported on the role of MIF in nerve recovery after ischemic stroke. The purpose of this study is to identify the molecular mechanisms of neuroprotection mediated by MIF.

Human Neurotrophin 4,NT-4 ELISA KIT

201-12-1293 96 tests
EUR 440
  • This Neurotrophin 4 ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Neurotrophin 4, NT-4 ELISA KIT

CSB-E04689h-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Human Neurotrophin 4, NT-4 in samples from serum, plasma, cell culture supernates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Neurotrophin 4, NT-4 ELISA KIT

1-CSB-E04689h
  • EUR 574.00
  • EUR 4013.00
  • EUR 2138.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Human Neurotrophin 4, NT-4 in samples from serum, plasma, cell culture supernates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Neurotrophin 4 (NT-4) CLIA Kit

abx197346-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Human NT-4(Neurotrophin-4) ELISA Kit

EH0246 96T
EUR 524.1
  • Detection range: 93.75-6000 pg/ml
  • Uniprot ID: P34130
  • Alias: NT-4/NT-5/NTF4/NTF5/GLC10/GLC1O/neurotrophic factor 4/neurotrophic factor 5/neurotrophin 4/neurotrophin 5(neurotrophin 4/5)/neurotrophin-4/neurotrophin-5/NT4//NT-4/5/NTF5NT5
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 56.25pg/ml

Human Neurotrophin 4,NT-4 ELISA KIT

CN-03258H1 96T
EUR 438

Human Neurotrophin 4,NT-4 ELISA KIT

CN-03258H2 48T
EUR 289

Human Neurotrophin 4(NT-4)ELISA Kit

GA-E1309HM-48T 48T
EUR 289

Human Neurotrophin 4(NT-4)ELISA Kit

GA-E1309HM-96T 96T
EUR 466

Human Neurotrophin 4(NT-4)ELISA Kit

QY-E01633 96T
EUR 361

Recombinant Human 4-1BB Receptor Protein

PROTQ07011-4 20ug
EUR 317
Description: 4-1BB Receptor, a member of the TNF superfamily of receptors, is mainly expressed on the surface of a variety of T cells, but also found in B cells, monocytes, and various transformed cell lines. 4-1BB Receptor binds to 4-1BBL to provide a co-stimulatory signal for T lymphocytes. Signaling by 4-1BB Receptor has been implicated in the antigen-presentation process and generation of cytotoxic T cells. The human 4-1BB Receptor gene codes for a 255 amino acid type I transmembrane protein containing a 17 amino acid N-terminal signal sequence, a 169 amino acid extracellular domain, a 27 amino acid transmembrane domain and a 42 amino acid cytoplasmic domain. Recombinant human soluble 4-1BB Receptor is a 167 amino acid polypeptide (17.7 kDa), which contains the cysteine rich TNFR-like extracellular domain of 4-1BB Receptor.

Recombinant Human PF-4 (CXCL4) Protein

PROTP02776-4 20ug
EUR 317
Description: PF-4 is a CXC chemokine that is expressed in megakaryocytes and stored in the α-granules of platelets. PF-4 is chemotactic towards neutrophils and monocytes and has been shown to inhibit angiogenesis. Recombinant human PF-4 is a 7.8 kDa protein containing 70 amino acid residues, including the four highly conserved residues present in CXC chemokines.

Feline IL-4 Recombinant Protein

R00230-4 5ug/vial
EUR 259
Description: IL-4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is a key regulator in humoral and adaptive immunity. Feline IL-4 Recombinant Protein is purified interleukin-4 produced in yeast.

Mouse Neurotrophin 4, NT-4 ELISA KIT

CSB-E04690m-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Mouse Neurotrophin 4, NT-4 in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse Neurotrophin 4, NT-4 ELISA KIT

1-CSB-E04690m
  • EUR 678.00
  • EUR 4644.00
  • EUR 2467.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Mouse Neurotrophin 4, NT-4 in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Neurotrophin 4, NT-4 ELISA KIT

CSB-E04691r-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Rat Neurotrophin 4, NT-4 in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat Neurotrophin 4, NT-4 ELISA KIT

1-CSB-E04691r
  • EUR 678.00
  • EUR 4644.00
  • EUR 2467.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Rat Neurotrophin 4, NT-4 in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Neurotrophin 4 (NT-4) CLIA Kit

abx197347-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Rat Neurotrophin 4 (NT-4) CLIA Kit

abx197348-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Rabbit neurotrophin 4,NT-4 ELISA Kit

CN-00681R1 96T
EUR 447

Rabbit neurotrophin 4,NT-4 ELISA Kit

CN-00681R2 48T
EUR 296

Rat Neurotrophin 4,NT-4 ELISA KIT

CN-01592R1 96T
EUR 464

Rat Neurotrophin 4,NT-4 ELISA KIT

CN-01592R2 48T
EUR 313

Mouse Neurotrophin 4,NT-4 ELISA KIT

CN-02428M1 96T
EUR 441

Mouse Neurotrophin 4,NT-4 ELISA KIT

CN-02428M2 48T
EUR 291

Mouse NT-4(Neurotrophin 4) ELISA Kit

EM1243 96T
EUR 524.1
  • Detection range: 15.625-1000 pg/ml
  • Uniprot ID: Q80VU4
  • Alias: NT-4//NT-5/NTF4/NTF5/GLC10/GLC1O/neurotrophic factor 4/neurotrophic factor 5/neurotrophin 4/neurotrophin 5(neurotrophin 4/5)/neurotrophin-4/neurotrophin-5/NT4//NT-4/5/NTF5NT5
Description: Method of detection: Double Antibody, Sandwich ELISA ;Reacts with: Mus ;Sensitivity: 9.375pg/ml

Rat NT-4(Neurotrophin 4) ELISA Kit

ER1201 96T
EUR 524.1
  • Detection range: 31.25-2000 pg/ml
  • Uniprot ID: P34131
  • Alias: NT-4/NT-5/NTF4/NTF5/GLC10/GLC1O/neurotrophic factor 4/neurotrophic factor 5/neurotrophin 4/neurotrophin 5(neurotrophin 4/5)/neurotrophin-4/Neurotrophin-5/neurotrophin-5/Neutrophic factor 4/N
  • Show more
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 18.75pg/ml

Rat Neurotrophin 4(NT-4)ELISA Kit

GA-E0552RT-48T 48T
EUR 317

Rat Neurotrophin 4(NT-4)ELISA Kit

GA-E0552RT-96T 96T
EUR 496

Mouse Neurotrophin 4(NT-4)ELISA Kit

GA-E0098MS-48T 48T
EUR 336

Mouse Neurotrophin 4(NT-4)ELISA Kit

GA-E0098MS-96T 96T
EUR 534

Rabbit neurotrophin 4,NT-4 ELISA Kit

GA-E0149RB-48T 48T
EUR 326

Rabbit neurotrophin 4,NT-4 ELISA Kit

GA-E0149RB-96T 96T
EUR 524

Rat Neurotrophin 4(NT-4)ELISA Kit

QY-E11100 96T
EUR 361

Mouse Neurotrophin 4(NT-4)ELISA Kit

QY-E21081 96T
EUR 361

Rabbit neurotrophin 4,NT-4 ELISA Kit

QY-E30150 96T
EUR 374

CLIA kit for Human NT-4 (Neurotrophin 4)

E-CL-H1172 1 plate of 96 wells
EUR 584
  • Gentaur's NT-4 CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NT-4 . Standards or samples are added to the micro CLIA plate wells and combined with the
  • Show more
Description: A sandwich CLIA kit for quantitative measurement of Human NT-4 (Neurotrophin 4) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human NT-4 (Neurotrophin 4)

E-EL-H1897 1 plate of 96 wells
EUR 534
  • Gentaur's NT-4 ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NT-4. Standards or samples are added to the micro ELISA plate wells and combined with th
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Human NT-4 (Neurotrophin 4) in samples from Serum, Plasma, Cell supernatant

CLIA kit for Mouse NT-4 (Neurotrophin 4)

E-CL-M0512 1 plate of 96 wells
EUR 584
  • Gentaur's NT-4 CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NT-4 . Standards or samples are added to the micro CLIA plate wells and combined with the
  • Show more
Description: A sandwich CLIA kit for quantitative measurement of Mouse NT-4 (Neurotrophin 4) in samples from Serum, Plasma, Cell supernatant

CLIA kit for Rat NT-4 (Neurotrophin 4)

E-CL-R0476 1 plate of 96 wells
EUR 584
  • Gentaur's NT-4 CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat NT-4 . Standards or samples are added to the micro CLIA plate wells and combined with the sp
  • Show more
Description: A sandwich CLIA kit for quantitative measurement of Rat NT-4 (Neurotrophin 4) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Mouse NT-4 (Neurotrophin 4)

E-EL-M0824 1 plate of 96 wells
EUR 534
  • Gentaur's NT-4 ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NT-4. Standards or samples are added to the micro ELISA plate wells and combined with th
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Mouse NT-4 (Neurotrophin 4) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Rat NT-4 (Neurotrophin 4)

E-EL-R0659 1 plate of 96 wells
EUR 534
  • Gentaur's NT-4 ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NT-4. Standards or samples are added to the micro ELISA plate wells and combined with the
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Rat NT-4 (Neurotrophin 4) in samples from Serum, Plasma, Cell supernatant

Recombinant Human Neurotrophin-4

7-01303 2µg Ask for price

Recombinant Human Neurotrophin-4

7-01304 10µg Ask for price

Recombinant Human Neurotrophin-4

7-01305 1mg Ask for price

NT-4 ELISA Kit| Rat Neurotrophin 4 ELISA Kit

EF017923 96 Tests
EUR 689

NT-4 ELISA Kit| Mouse Neurotrophin 4 ELISA Kit

EF013791 96 Tests
EUR 689

Human Neurotrophin-4 (NT-4) ELISA Kit, 96 tests, Quantitative

9695 1 Kit
EUR 773

Human FibrOut 4, for brain, neural

4-21552 1 ml Ask for price

Human FibrOut 4, for brain, neural

4-21553 5 x 1 ml Ask for price

Individual Reaction Mix 4

G065-4 200 reactions
EUR 167

NT-4, human recombinant

4309-10
EUR 256

NT-4, human recombinant

4309-1000
EUR 5106

NT-4, human recombinant

4309-50
EUR 697

Recombinant Human NT-4 Protein

PROTP34130-1 10ug
EUR 317
Description: NT-4 is a neurotrophic factor structurally related to β-NGF, BDNF, and NT-3. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. NT-4 is expressed in the prostate, thymus, placenta and skeletal muscle. NT-4 can signal through the LNGFR and trkB receptors and promotes the survival of peripheral sensory sympathetic neurons. Recombinant human NT-4 is a noncovalently linked homodimer, of two 14.0 kDa polypeptide monomers (260 total amino acid residues).

pLenti-CLDN1 shRNA-4 Plasmid

PVTBAV04867-4 2 ug
EUR 356

Recombinant Neurotrophin 4 (NT4)

4-RPA107Hu01
  • EUR 512.16
  • EUR 240.00
  • EUR 1645.60
  • EUR 615.20
  • EUR 1130.40
  • EUR 406.00
  • EUR 3964.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P34130
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 17.7kDa
  • Isoelectric Point: 9.1
Description: Recombinant Human Neurotrophin 4 expressed in: E.coli

Recombinant Neurotrophin 4 (NT4)

4-RPA107Mu01
  • EUR 501.41
  • EUR 237.00
  • EUR 1605.28
  • EUR 601.76
  • EUR 1103.52
  • EUR 398.00
  • EUR 3863.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q80VU4
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 17.7kDa
  • Isoelectric Point: 9.3
Description: Recombinant Mouse Neurotrophin 4 expressed in: E.coli

DIGITAL SHAKER FOR 4 MICROPLATES, 120V

6780-4 1/pk
EUR 798
Description: Lab Equipment; Shakers & Mixers

Mouse FibrOut 4, for brain, neural

4-20507 1 ml Ask for price

Mouse FibrOut 4, for brain, neural

4-20508 5 x 1 ml Ask for price

Rat FibrOut 4, for brain, neural

4-20533 1 ml Ask for price

Rat FibrOut 4, for brain, neural

4-20534 5 x 1 ml Ask for price

NT-4, Human

HY-P7272 50ug
EUR 762

NT-4 (human)

GT15001 100 ug
EUR 526

Bone Dissociation System 4 (Osteoblasts), Adult Mouse

4-20214 ea Ask for price

Liver Dissociation System 4 (Hepatocytes, rat), Rat

4-20304 ea Ask for price

Pituitary Dissociation System 4 (Pituitary), Adult mouse

4-20404 ea Ask for price

Thymus Dissociation System 4 (Epithelial), Adult rat

4-20444 ea Ask for price

ExoStd? Lyophilized Exosome Standard (30 µg, Human Plasma, 4 vials)

M1040-4
EUR 909

ExoStd? Lyophilized Exosome Standard (100 µg, Human Plasma, 4 vials)

M1041-4
EUR 1137

ExoStd? Lyophilized Exosome Standard (30 µg, Human Serum, 4 vials)

M1042-4
EUR 909

ExoStd? Lyophilized Exosome Standard (100 µg, Human Serum, 4 vials)

M1043-4
EUR 1132

ExoStd? Lyophilized Exosome Standard (30 µg, Human Urine, 4 vials)

M1044-4
EUR 909

ExoStd? Lyophilized Exosome Standard (100 µg, Human Urine, 4 vials)

M1045-4
EUR 1137

ExoStd? Lyophilized Exosome Standard (30 µg, Human Saliva, 4 vials)

M1046-4
EUR 914

ExoStd? Lyophilized Exosome Standard (100 µg, Human Saliva, 4 vials)

M1047-4
EUR 1137

DIGITAL SHAKER FOR 4 MICROPLATES, 230V-EU PLUG

6781-4 1/pk
EUR 798
Description: Lab Equipment; Shakers & Mixers

DIGITAL SHAKER FOR 4 MICROPLATES, 230V-UK PLUG

6782-4 1/pk
EUR 798
Description: Lab Equipment; Shakers & Mixers

Cartilage Dissociation System 4 (Chondrocytes), Mouse and Rat

4-20234 ea Ask for price

Mammary Dissociation System 4 (Epithelial, Swiss 3T3), Mouse

4-20324 ea Ask for price

Pancreas Dissociation System 4 (Islets), Mouse and Rat

4-20374 ea Ask for price

Skin Dissociation System 4 (Keratinocytes), Mouse and Rat

4-20434 ea Ask for price

NT-4/ Rat NT- 4 ELISA Kit

ELA-E0107r 96 Tests
EUR 886

Human Neurotrophin 4 (NT4) Protein

20-abx167127
  • EUR 718.00
  • EUR 286.00
  • EUR 2221.00
  • EUR 857.00
  • EUR 509.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Human Neurotrophin 4 (NTF4) Protein

20-abx262207
  • EUR 328.00
  • EUR 5826.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

anti-Neurotrophin 4

YF-PA13491 50 ul
EUR 363
Description: Mouse polyclonal to Neurotrophin 4

anti-Neurotrophin 4

YF-PA13492 100 ul
EUR 403
Description: Rabbit polyclonal to Neurotrophin 4

anti-Neurotrophin 4

YF-PA13493 100 ug
EUR 403
Description: Rabbit polyclonal to Neurotrophin 4

anti-Neurotrophin 4

YF-PA24273 50 ul
EUR 334
Description: Mouse polyclonal to Neurotrophin 4

Human Neurotrophin-4 (NTF4)

1-CSB-RP099294h
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 17.9 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human Neurotrophin-4(NTF4) expressed in E.coli

Adipose/Fat Dissociation System 4 (Predipocytes), Mouse and Rat

4-20194 ea Ask for price

Brain Dissociation System 4 (Endothelial, Microvessels), Mouse and Rat

4-20224 ea Ask for price

Endothelial Dissociation System 4 (Endothelial,Cerebral), Mouse and Rat

4-20244 ea Ask for price

Heart Dissociation System 4 (Myocytes,Ventricles), Mouse and Rat

4-20274 ea Ask for price

Muscle Dissociation System 4 (Smooth muscle), Mouse and Rat

4-20334 ea Ask for price

Human cell neuroblastoma incubated in a modified media Eagle Dulbecco under lack of oxygen-glucose (OGD) for 4 hours and then returned to the aerobic environment normal to reperfusion (OGD / R). 30 ng / mL MIF recombinant (30 ng / mL) or ISO-1 (MIF antagonist; 50 M) was administered to the Human neuroblastoma cells. Then the cell culture is assigned to one of four groups: control, OGD / R, OGD / R by MIF, OGD / R with ISO-1.