Neuroinflammation play an important role in the pathogenesis of germinal matrix hemorrhage (GMH). neurotrophin – 4 (NT- 4 ) is a member of the neurotrophin family and interact with tropomyosin receptor kinase B (TrkB ). NT- 4 has been shown to provide the following neurological effects of cerebral ischemia. We aimed to determine the function of the nerves of NT- 4 -TrkB signaling, as well as the signaling cascade downstream of phosphatidylinositol-3-kinase (PI3K) / protein kinase B (Akt) / forkhead box protein O1 (FoxO1), the following GMH in neonatal rats.
GMH induced by intraparenchymal injection of bacterial collagenase (0.3 U) in P7 rat pup. A total of 163 pups were used in this study. Recombinant Human NT- 4 is given intranasally at 1 hour after injection of collagenase. Selective TrkB ANA-12 antagonist, selective PI3K inhibitor LY29 4 002, and FoxO1 activate CRISPR given intracerebroventricularly at 2 4 hours before NT- 4 treatment to investigate the underlying mechanisms. Short-term and long-term neurobehavioral assessment, immunofluorescence staining, Nissl’s staining and Western blot performed.
The expression of phosphorylated TrkB increased after GMH, reaching peak levels on day 3 after bleeding. TrkB receptors were observed in neurons, microglia, and astrocytes. Administration rh-NT- 4 induced phosphorylation of TrkB, the expression of PI3K and Akt phosphorylation. Meanwhile, the decrease in FoxO1 and IL-6 levels. Selective inhibition of TrkB / PI3K / Akt signaling in microglia increase expression levels FoxO1 and pro-inflammatory cytokines. FoxO1 activate CRISPR increased expression of IL-6, suggesting that perhaps FoxO1 potential inducer of pro-inflammatory factors.
These results demonstrate that the PI3K / Akt / FoxO1 downstream signaling pathways may TrkB activation. Rat pups treated with rh-NT- 4 performed better than vehicle-treated animals both short-term and long-term behavioral tests. These data indicate that rh-NT – 4 reducing the expression of pro-inflammatory cytokines, improve neurological function, attenuated neuroinflammation, and thus reduced post-hemorrhagic hydrocephalus after GMH by TrkB / PI3K / Akt / FoxO1 track. These results indicate that rh-NT- 4 could be a promising therapeutic strategy for repairing nerve inflammation and hydrocephalus after GMH or other similar brain injuries.
Brain-derived neurotrophic factors mediate macrophage migration inhibitory factor to protect neurons against oxygen-glucose deficiency.
Macrophage migration inhibitory factor (MIF) is a chemokine that plays an important role in immune system function. Previous research suggests that MIF protect neurons under ischemic conditions. However, several studies reported on the role of MIF in nerve recovery after ischemic stroke. The purpose of this study is to identify the molecular mechanisms of neuroprotection mediated by MIF.
Recombinant Human Neurotrophin-4 |
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7-01303 | CHI Scientific | 2µg | Ask for price |
Recombinant Human Neurotrophin-4 |
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7-01304 | CHI Scientific | 10µg | Ask for price |
Recombinant Human Neurotrophin-4 |
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7-01305 | CHI Scientific | 1mg | Ask for price |
Recombinant Human Neurotrophin-4 |
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AP60103 | SAB | 100ug | EUR 1554 |
Recombinant Human Neurotrophin-4 |
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cyt-626-10g | ProSpec Tany | 10µg | EUR 145 |
Recombinant Human Neurotrophin-4 |
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cyt-626-1mg | ProSpec Tany | 1mg | EUR 4680 |
Recombinant Human Neurotrophin-4 |
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cyt-626-2g | ProSpec Tany | 2µg | EUR 60 |
Neurotrophin-4 Human Recombinant |
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rAP-2701 | Angio Proteomie | Inquiry | Ask for price |
Recombinant Human Neurotrophin-3/NGF-2/NT-3 Protein |
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RP00496 | Abclonal | 10 μg | EUR 286 |
NT-3 Neurotrophin-3 Mouse Recombinant Protein |
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PROTP20181 | BosterBio | Regular: 10ug | EUR 380.4 |
Description: Neurotrophin-3 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 119 amino acids and having a molecular mass of 13.6kDa. ;The NT-3 is purified by proprietary chromatographic techniques. |
Recombinant Human Neurotrophin-4(NTF4) |
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AP72096 | SAB | 1mg | EUR 1978 |
Recombinant Human NT-3/Neurotrophin-3/NTF3 Protein, No Tag |
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E40KMH1479 | EnoGene | 20ug | EUR 495 |
NNT1 Neurotrophin-1 Human Recombinant Protein |
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PROTQ9UBD9-1 | BosterBio | Regular: 10ug | EUR 380.4 |
Description: Neurotrophin-1 Human Recombinant (28-225) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 199 amino acids and having a molecular mass of 22kDa.;The NNT-1 is purified by proprietary chromatographic techniques. |
Genorise® Recombinant Equine Neurotrophin-4 protein |
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GR104319 | Genorise Scientific | 5 µg | EUR 285 |
Neurotrophin-3 Recombinant Protein |
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91-065 | ProSci | 0.05 mg | EUR 821.4 |
Description: Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors and is structurally related to beta -NGF, BDNF and NT-4. The NT3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT3.The amino acid sequences of mature human, murine and rat NT-3 are identical. NT-3 selectively promotes the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. |
Recombinant Human Neurotrophin-4 (NTF4), partial |
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CSB-EP016121HU1 | Cusabio | 1830 mg | Ask for price |
Recombinant Neurotrophin 4 (NT4) |
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RPU53898-100ug | Biomatik Corporation | 100ug | EUR 566.5 |
Recombinant Neurotrophin 4 (NT4) |
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RPU53898-1mg | Biomatik Corporation | 1mg | EUR 2511.6 |
Recombinant Neurotrophin 4 (NT4) |
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RPU53898-50ug | Biomatik Corporation | 50ug | EUR 455.4 |
Recombinant Neurotrophin 4 (NT4) |
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RPU43034-100ug | Biomatik Corporation | 100ug | EUR 552.2 |
Recombinant Neurotrophin 4 (NT4) |
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RPU43034-1mg | Biomatik Corporation | 1mg | EUR 2446.6 |
Recombinant Neurotrophin 4 (NT4) |
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RPU43034-50ug | Biomatik Corporation | 50ug | EUR 443.3 |
Recombinant Neurotrophin 4 (NT4) |
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RPU59108-100ug | Biomatik Corporation | 100ug | EUR 1044.4 |
Recombinant Neurotrophin 4 (NT4) |
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RPU59108-1mg | Biomatik Corporation | 1mg | EUR 4083.3 |
Recombinant Neurotrophin 4 (NT4) |
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RPU59108-50ug | Biomatik Corporation | 50ug | EUR 652.6 |
Recombinant Neurotrophin 4 (NT4) |
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RPU56286-100ug | Biomatik Corporation | 100ug | EUR 775.5 |
Recombinant Neurotrophin 4 (NT4) |
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RPU56286-1mg | Biomatik Corporation | 1mg | EUR 3057.6 |
Recombinant Neurotrophin 4 (NT4) |
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RPU56286-50ug | Biomatik Corporation | 50ug | EUR 470.4 |
Recombinant Neurotrophin 4 (NT4) |
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RPA107Hu01 | Cloud-Clone | 10ug | EUR 184 |
Recombinant Neurotrophin 4 (NT4) |
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4-RPA107Hu01 | Cloud-Clone |
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Description: Recombinant Human Neurotrophin 4 expressed in: E.coli |
Recombinant Neurotrophin 4 (NT4) |
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RPA107Mu01 | Cloud-Clone | 10ug | EUR 179 |
Recombinant Neurotrophin 4 (NT4) |
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4-RPA107Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Neurotrophin 4 expressed in: E.coli |
Recombinant Neurotrophin 4 (NT4) |
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RPA107Ra01 | Cloud-Clone | 10ug | EUR 196 |
Genorise® recombinant Bovine Neurotrophin 4 protein |
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GR120051 | Genorise Scientific | 5 µg | EUR 285 |
Genorise® recombinant Canine Neurotrophin 4 protein |
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GR122076 | Genorise Scientific | 5 µg | EUR 285 |
Recombinant Human Neurotrophin-3/NTF3 Protein (Active) |
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PKSH032803-10ug | Elabscience Biotech | 10ug | EUR 178 |
Description: Human |
Recombinant Human Neurotrophin-3/NTF3 Protein (Active) |
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PKSH032803-50ug | Elabscience Biotech | 50ug | EUR 523 |
Description: Human |
Genorise® Recombinant feline Neurotrophin 3 (NT-3) protein |
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GR186035 | Genorise Scientific | 5 mg | EUR 285 |
Genorise® recombinant Bovine Neurotrophin 3 (NT-3) protein |
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GR120050 | Genorise Scientific | 5 µg | EUR 285 |
Human Neurotrophin-3 Recombinant Protein Tag Free Lyophilized |
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IHUNT3RTFLY50UG | Innovative research | each | EUR 988 |
Description: Human Neurotrophin-3 Recombinant Protein Tag Free Lyophilized |
Human Neurotrophin-3 (Recombinant) |
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22060430-1 | Glycomatrix | 2 µg | EUR 146.42 |
Recombinant Human Neurotrophin 3/NTF3 protein (His tag) |
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PDEH100226-100ug | Elabscience Biotech | 100ug | EUR 118 |
Description: Human |
Recombinant Human Neurotrophin 3/NTF3 protein (His tag) |
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PDEH100226-20ug | Elabscience Biotech | 20ug | EUR 68 |
Description: Human |
Neurotrophin-3, recombinant (Human) |
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033-29 | PHOENIX PEPTIDE | 10 μg | EUR 390.96 |
Recombinant Human Neurotrophin-3 |
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7-01294 | CHI Scientific | 2µg | Ask for price |
Recombinant Human Neurotrophin-3 |
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7-01295 | CHI Scientific | 10µg | Ask for price |
Recombinant Human Neurotrophin-3 |
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7-01296 | CHI Scientific | 1mg | Ask for price |
Recombinant Human Neurotrophin-3 |
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cyt-257-10g | ProSpec Tany | 10µg | EUR 145 |
Recombinant Human Neurotrophin-3 |
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cyt-257-1mg | ProSpec Tany | 1mg | EUR 4680 |
Recombinant Human Neurotrophin-3 |
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cyt-257-2g | ProSpec Tany | 2µg | EUR 60 |
Recombinant Human Neurotrophin-1 |
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cyt-869-10g | ProSpec Tany | 10µg | EUR 145 |
Recombinant Human Neurotrophin-1 |
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cyt-869-1mg | ProSpec Tany | 1mg | EUR 4680 |
Recombinant Human Neurotrophin-1 |
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cyt-869-2g | ProSpec Tany | 2µg | EUR 60 |
Neurotrophin-3 Human Recombinant |
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rAP-2697 | Angio Proteomie | Inquiry | Ask for price |
Neurotrophin-1 Human Recombinant |
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rAP-2707 | Angio Proteomie | Inquiry | Ask for price |
NT-4, Neurotrophin-4, human |
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RC218-15 | Bio Basic | 2ug | EUR 125.26 |
Recombinant Human Neurotrophin-3/NT3 |
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AP74825 | SAB | 1mg | EUR 3209 |
Recombinant Human Neurotrophin-3/NT3 |
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C079-10ug | Novoprotein | 10ug | EUR 242.4 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2. |
Recombinant Human Neurotrophin-3/NT3 |
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C079-1mg | Novoprotein | 1mg | EUR 2739.6 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2. |
Recombinant Human Neurotrophin-3/NT3 |
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C079-500ug | Novoprotein | 500ug | EUR 1910.4 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2. |
Recombinant Human Neurotrophin-3/NT3 |
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C079-50ug | Novoprotein | 50ug | EUR 595.2 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2. |
Recombinant Human Neurotrophin-3 (NTF3) |
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CSB-EP016120HU | Cusabio | 7990 mg | Ask for price |
Recombinant Human Neurotrophin-3 (NTF3) |
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CSB-MP016120HU | Cusabio | 7843 mg | Ask for price |
Recombinant Human Neurotrophin-3 (NTF3) |
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CSB-BP016120HU | Cusabio | 7886 mg | Ask for price |
Recombinant Human Neurotrophin-3 (NTF3) |
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RPC27838-100ug | Biomatik Corporation | 100ug | EUR 1463 |
Recombinant Human Neurotrophin-3 (NTF3) |
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RPC27838-1mg | Biomatik Corporation | 1mg | EUR 6879.6 |
Recombinant Human Neurotrophin-3 (NTF3) |
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RPC27838-20ug | Biomatik Corporation | 20ug | EUR 550 |
Recombinant Human Neurotrophin-3 (NTF3) |
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RPC28701-100ug | Biomatik Corporation | 100ug | EUR 1170.4 |
Recombinant Human Neurotrophin-3 (NTF3) |
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RPC28701-1mg | Biomatik Corporation | 1mg | EUR 3252 |
Recombinant Human Neurotrophin-3 (NTF3) |
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RPC28701-20ug | Biomatik Corporation | 20ug | EUR 440 |
Recombinant Human Neurotrophin-3 (NTF3) |
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RPC28729-100ug | Biomatik Corporation | 100ug | EUR 528 |
Recombinant Human Neurotrophin-3 (NTF3) |
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RPC28729-1mg | Biomatik Corporation | 1mg | EUR 2412.5 |
Recombinant Human Neurotrophin-3 (NTF3) |
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RPC28729-20ug | Biomatik Corporation | 20ug | EUR 295.7 |
Genorise® recombinant Sheep Neurotrophin 4 |
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GR123022 | Genorise Scientific | 5 µg | EUR 285 |
Human recombinant Neurotrophins-3 (NT-3) purified protein |
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NT3-R02 | Alpha Diagnostics | 2 ug | EUR 270 |
Human recombinant Neurotrophins-3 (NT-3) purified protein |
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NT3-R10 | Alpha Diagnostics | 10 ug | EUR 489.6 |
Human recombinant Neurotrophins-3 (NT-3) purified protein |
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NT3-R100 | Alpha Diagnostics | 100 ug | EUR 2690.4 |
Human recombinant Neurotrophins-3 (NT-3) purified protein |
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NT3-R1000 | Alpha Diagnostics | 1 mg | EUR 9998.4 |
Recombinant Human NTF3/ Neurotrophin 3 Protein, Untagged, E.coli-10ug |
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QP1246-10ug | EnQuireBio | 10ug | EUR 284.4 |
Recombinant Human NTF3/ Neurotrophin 3 Protein, Untagged, E.coli-1mg |
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QP1246-1mg | EnQuireBio | 1mg | EUR 5942.4 |
Recombinant Human NTF3/ Neurotrophin 3 Protein, Untagged, E.coli-2ug |
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QP1246-2ug | EnQuireBio | 2ug | EUR 163.2 |
Recombinant Human Precursor Neurotrophin-3 |
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7-01297 | CHI Scientific | 2µg | Ask for price |
Recombinant Human Precursor Neurotrophin-3 |
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7-01298 | CHI Scientific | 10µg | Ask for price |
Recombinant Human Precursor Neurotrophin-3 |
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7-01299 | CHI Scientific | 1mg | Ask for price |
Genorise® Recombinant Equine Neurotrophin-3 protein |
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GR104318 | Genorise Scientific | 5 µg | EUR 285 |
Recombinant Human NTF3/ Neurotrophin 3 Protein, Untagged, E.coli-100ug |
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QP1246-100ug | EnQuireBio | 100ug | EUR 849.6 |
Recombinant Human NTF3/ Neurotrophin 3 Protein, Untagged, E.coli-500ug |
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QP1246-500ug | EnQuireBio | 500ug | EUR 3645.6 |
Recombinant Human Neurotrophin-3 (NTF3) (Active) |
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CSB-AP003801HU | Cusabio | 8580 mg | Ask for price |
Genorise® recombinant Canine Neurotrophin 3 protein |
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GR122075 | Genorise Scientific | 5 µg | EUR 285 |
Neurotrophin-3, recombinant (Human) - I-125 Labeled |
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T-033-29 | PHOENIX PEPTIDE | 10 μCi | EUR 1145.88 |
Recombinant Mouse Neurotrophin-3 |
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7-01300 | CHI Scientific | 2µg | Ask for price |
Recombinant Mouse Neurotrophin-3 |
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7-01301 | CHI Scientific | 10µg | Ask for price |
Recombinant Mouse Neurotrophin-3 |
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7-01302 | CHI Scientific | 1mg | Ask for price |
Recombinant Mouse Neurotrophin-3 |
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cyt-688-10g | ProSpec Tany | 10µg | EUR 145 |
Recombinant Mouse Neurotrophin-3 |
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cyt-688-1mg | ProSpec Tany | 1mg | EUR 4680 |
Recombinant Mouse Neurotrophin-3 |
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cyt-688-2g | ProSpec Tany | 2µg | EUR 60 |
Neurotrophin-3 Mouse Recombinant |
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rAP-2702 | Angio Proteomie | Inquiry | Ask for price |
Human NT-4(Neurotrophin-4) ELISA Kit |
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EH0246 | FN Test | 96T | EUR 628.92 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 56.25pg/ml |
Recombinant Neurotrophin 3 (NT3) |
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RPU43032-100ug | Biomatik Corporation | 100ug | EUR 530.2 |
Recombinant Neurotrophin 3 (NT3) |
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RPU43032-1mg | Biomatik Corporation | 1mg | EUR 2347.8 |
Recombinant Neurotrophin 3 (NT3) |
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RPU43032-50ug | Biomatik Corporation | 50ug | EUR 425.7 |
Recombinant Neurotrophin 3 (NT3) |
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RPU43033-100ug | Biomatik Corporation | 100ug | EUR 530.2 |
Recombinant Neurotrophin 3 (NT3) |
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RPU43033-1mg | Biomatik Corporation | 1mg | EUR 2347.8 |
Recombinant Neurotrophin 3 (NT3) |
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RPU43033-50ug | Biomatik Corporation | 50ug | EUR 425.7 |
Recombinant Neurotrophin 3 (NT3) |
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RPU56113-100ug | Biomatik Corporation | 100ug | EUR 554.4 |
Recombinant Neurotrophin 3 (NT3) |
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RPU56113-1mg | Biomatik Corporation | 1mg | EUR 2184 |
Recombinant Neurotrophin 3 (NT3) |
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RPU56113-50ug | Biomatik Corporation | 50ug | EUR 336 |
Recombinant Neurotrophin 3 (NT3) |
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RPU56654-100ug | Biomatik Corporation | 100ug | EUR 470.4 |
Recombinant Neurotrophin 3 (NT3) |
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RPU56654-1mg | Biomatik Corporation | 1mg | EUR 2184 |
Recombinant Neurotrophin 3 (NT3) |
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RPU56654-50ug | Biomatik Corporation | 50ug | EUR 385 |
Recombinant Neurotrophin 3 (NT3) |
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RPU55489-100ug | Biomatik Corporation | 100ug | EUR 728.2 |
Recombinant Neurotrophin 3 (NT3) |
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RPU55489-1mg | Biomatik Corporation | 1mg | EUR 2870.4 |
Recombinant Neurotrophin 3 (NT3) |
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RPU55489-50ug | Biomatik Corporation | 50ug | EUR 441.6 |
Recombinant Neurotrophin 3 (NT3) |
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RPA106Hu01 | Cloud-Clone | 10ug | EUR 172 |
Recombinant Neurotrophin 3 (NT3) |
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4-RPA106Hu01 | Cloud-Clone |
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Description: Recombinant Human Neurotrophin 3 expressed in: E.coli |
Recombinant Neurotrophin 3 (NT3) |
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RPA106Hu02 | Cloud-Clone | 10ug | EUR 172 |
Recombinant Neurotrophin 3 (NT3) |
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4-RPA106Hu02 | Cloud-Clone |
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Description: Recombinant Human Neurotrophin 3 expressed in: E.coli |
Recombinant Neurotrophin 3 (NT3) |
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RPA106Mu01 | Cloud-Clone | 10ug | EUR 140 |
Recombinant Neurotrophin 3 (NT3) |
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RPA106Mu02 | Cloud-Clone | 10ug | EUR 140 |
Recombinant Neurotrophin 3 (NT3) |
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RPA106Ra01 | Cloud-Clone | 10ug | EUR 184 |
Human cell neuroblastoma incubated in a modified media Eagle Dulbecco under lack of oxygen-glucose (OGD) for 4 hours and then returned to the aerobic environment normal to reperfusion (OGD / R). 30 ng / mL MIF recombinant (30 ng / mL) or ISO-1 (MIF antagonist; 50 M) was administered to the Human neuroblastoma cells. Then the cell culture is assigned to one of four groups: control, OGD / R, OGD / R by MIF, OGD / R with ISO-1.